MS03-P01 BioSAXS at ESRF: The solution scattering Beamline BM29 Martha Brennich (EMBL Grenoble, Grenoble, France) Pernot Petra (ESRF, Grenoble, France) Mark Tully (ESRF, Grenoble, France)email: brennich@embl.frSmall angle X-ray scattering (SAXS) is ideally suited to study the structure and structural changes of biological macromolecules in solution. The last decade has seen a surge of technological and conceptual development in small angle scattering that increased the importance of SAXS in the toolkit of structural biology. At the ESRF, the BioSAXS beamline BM29 is dedicated to solution scattering and optimized for routine measurements of biological macromolecules [1].  It aims at facilitating SAS for non-experts by providing easy and rapid access as well as reducing the effort to collect and assess data.
BM29 primarily offers two data collection modes: BioSAXS with a robotic liquid handling sample changer (SC) and BioSAXS with online size-exclusion chromatography (SEC). Both modes are highly automated and switching between modes is instantaneous. In both modes, primary data processing from azimuthal integration to determination of SAS invariants is completely automated and provides immediate feedback. The electronic logbook ISPyB collects all data and associates relevant meta-data. It assists the users in designing their experiments, in associating data with meta-data and in the primary assessment of data quality.
Here the experimental setups available on BM29 will be described together with the various examples of the BioSAXS data obtained. Recent examples of BM29 research, including the integration of microfluidics systems and online ion-exchange chromatography as well as the possibilities for new experiments in BioSAXS.
 
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[1] P. Pernot et al., J. Syn. Rad. 20 (2013) 660-664.
Keywords: SAXS, complementary techniques, instrumentation