MS11-P04 The crystal structure of the CARD-CARD disk of the human apoptosome and its structural insights into the assembly of the death-domain fold Su-Chang Lin (Genomics Research Center, Academia Sinica, Taipei, Taiwan) Yu-Chih Lo (Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Taipei, Taiwan) Tsung-Wei Su (Genomics Research Center, Academia Sinica, Taipei, Taiwan)email: tomlin@gate.sinica.edu.twThe caspase recruitment domain (CARD) of the human apoptosome recruits caspase-9 CARD via the homotypic interactions for caspase activation in apoptosis. Here we present the crystal structure of the CARD-CARD disk of the human apoptosome and also show the difference in the CARD assembly between the Caenorhabditis elegans, Drosophila, and human apoptosomes, which suggests that the helical assembly only exists in the vertabtate DD-folds.  We also found that of the death-domain folds have five different assembling mechanisms for their specific roles in signal transduction.
 
References:

[1] Su, TW et. al. (2017). Structure, 25, 407-420.

[2] Kao WP et. al. (2015) Apoptosis. 20. 174-195
Keywords: Apoptosome, assembly, death domain