MS40-P02 A high-throughput crystallisation facility interacting with the new VMXi beamline at the Diamond Light Source Halina Mikolajek (Diamond Light Source, Didcot, United Kingdom) James Sandy (Diamond Light Source, Didcot, United Kingdom) Juan Sanchez-Weatherby (Diamond Light Source, Didcot, United Kingdom) Thomas Sorensen (Diamond Light Source, Didcot, United Kingdom)email: halina.mikolajek@diamond.ac.ukA new state of the art crystallisation platform for soluble and membrane proteins has been created within the Research Complex at Harwell combining the expertise of Diamond Light Source, MPL and PPUK. This platform is high-throughput and provides users with an easy route into the VMXi beamline, the new fully automated in situ and serial crystallography beamline at the Diamond Light Source, UK. Users from all over the world have access to this facility and can also deliver their samples for crystallisation experiments. Crystallisation can be set up and stored at 4°C as well as at room temperature with humidity control and in lipid cubic phase (LCP) in a number of different plate types. A large number of commercial screens are available and customised screens can be designed and prepared easily. The crystallisation experiments are imaged using Formulatrix Rock Imagers and can be viewed via Rock Maker as well as SynchWeb, which is available on the VMXi beamline. Users can evaluate their crystallisation hits on the VMXi beamline and cycle faster than ever between optimisation cycles to obtain their molecular structure.     
 
References:

Keywords: Crystallisation, Facility, VMXi