MS05-P11 Novel Fluorescent Probes for Retinoic Acid binding proteins Charles Tomlinson (Department of Chemistry, Durham University, Durham, United Kingdom) Ehmke Pohl (Department of Chemistry, Durham University, Durham, United Kingdom) David Chisholm (Department of Chemistry, Durham University, Durham, United Kingdom) Andy Whiting (Department of Chemistry, Durham University, Durham, United Kingdom)email: c.w.e.tomlinson@durham.ac.ukDevelopment of new, fluorescent, retinoic acid analogues offers the opportunity to investigate retinoic acid binding proteins both in vitro and in vivo. Retinoic acid signaling is vital for normal growth and development, and offers an attractive target for novel therapies, particularly within the field of neurodegenerative disease. Through different binding affinities the capability of, and variation between, different protein isoforms can be investigated, and potentially harnessed to design isoform-specific ligands. The fluorescent nature of the probes, and their enhanced stability over that of retinoic acid, makes them ideal for following in vitro via fluorescence spectroscopy and will be invaluable for high throughput ligand screening - feeding into the structure based design of future ligands.  
 
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Keywords: Retinoids, Binding, Fluoresence