MS04-P02 Synthesis and Characterization of Cross-Linked Lysozyme Crystals filled with Single-Walled Carbon Nanotubes Bionanomaterials Guillermo Escolano (Departamento de Química Orgánica, Facultad de Ciencias (UGR) and Laboratorio de estudios cristalográficos, Instituto Andaluz de Ciencias de la Tierra (CSIC-UGR), Granada, Spain) Rafael Contreras-Montoya (Departamento de Química Orgánica, Facultad de Ciencias (UGR), Granada, Spain) Juan J. Díaz-Mochón (Departamento de Química Farmacéutica y Orgánica, Facultad de Farmacia, (UGR), Centre for Genomics and Oncological Research, Pfizer/UGR/Andalusian Regional Goverment, PTS Granada, Granada, Spain) Luis Álvarez de Cienfuegos (Departamento de Química Orgánica, Facultad de Ciencias (UGR), Granada, Spain) José A. Gavira (Laboratorio de estudios cristalográficos, Instituto Andaluz de Ciencias de la Tierra (CSIC-UGR), Granada, Spain)email: gec@ugr.esNovel bionanomaterials are hybrid materials that include the combination of biomolecules and inorganic substances to generate, enhance or support relevant properties. Bionanomaterials have useful applications in bio- and nanotechnology applications 1,2 .Among the biomolecules used to prepare hybrid materials, proteins have shown to be versatile materials thank to their capacity to self-assembly in crystalline form generating a porous network of nanometer size. The internal cavities of the protein have the ability to act as template3, 4 and it gives the material the possibility to extrapolate nanoscale properties to macroscopic materials for practical applications.
In this work, we present a new methodology to homogenously incorporate inorganic particles within protein crystals using dipeptide hydrogels as growth media. To exemplify this methodology, we have obtained lysozyme crystals incorporating single wallet carbon nanotubes at different concentration. Crystals were grown in Fmoc-PhePhe-OH hydrogels5. The influence of the nanotubes on the diffraction properties, hardness, enzymatic activity and conductivity will be presented and discussed, as well as a full characterization of these new materials.


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[3] M. Liang, L. Wang, R. Su, W. Qi, M. Wang, Y. Yua and Z. He. Synthesis of silver nanoparticles within cross-linked lysozyme crystals as recyclable catalysts for 4-nitrophenol reduction. Catal. Sci. Technol., 2013, 3, 1910-1914.

[4] O. L. Muskens, M. W. England, L. Danos, M. Li, and S. Mann Plasmonic Response of Ag- and Au-Infiltrated Cross-Linked Lysozyme Crystals. Adv. Funct. Mater. 2013, 23, 281–290.

[5] Conejero-Muriel, et al, Chem. Commun. 51, (2015), 3862 & M. Conejero-Muriel, et al., CrystEngComm, 17, (2015), 8072.
Keywords: bionanomaterials, crystallization, carbon nanotubes